Eastern Conference
Atlantic Division
- Carolina Dynamo
- Charleston Battery
- Hampton Roads Mariners
- Hershey Wildcats
- Jacksonville Cyclones
- Raleigh Flyers
- Richmond Kickers
Northeast Division
- Connecticut Wolves
- Long Island Rough Riders
- Montreal Impact
- Rochester Rhinos
- Toronto Lynx
- Worcester Wildfire
Western Conference
Central Division
- Atlanta Ruckus
- Milwaukee Rampage
- Minnesota Thunder
- Nashville Metros
- New Orleans Riverboat Gamblers
- Orlando Sundogs
Pacific Division
- Arizona Aztecs
- California Jaguars
- Colorado Foxes
- El Paso Patriots
- Orange County
- Seattle Sounders
- Vancouver 86ers
Each team will play against all its conference rivals at home and away. In addition teams will play four additional games, against either Eastern of Western Conference foes. The formats for the U.S. Open Cup and the A-League playoffs have yet to be determined.