Here is the 1999 Tampa Bay Mutiny schedule. Please note that all times are ET and that the schedule is subject to change.
| Date | Opponent | Time (ET) |
| Sat. March 20 | D.C. United | 7:00 p.m. |
| Sat. April 3 | Columbus | 7:00 p.m. |
| Sun. April 11 | MetroStars | 5:00 p.m. |
| Sun. April 18 | at Dallas | 5:00 p.m. |
| Sun. April 25 | at MetroStars | 1:00 p.m. |
| Sat. May 1 | Miami | 7:00 p.m. |
| Sat. May 8 | Chicago | 7:00 p.m. |
| Sat. May 15 | at Los Angeles | 10:30 p.m. |
| Sat. May 22 | at Columbus | 7:30 p.m. |
| Sat. May 29 | at Miami | 7:30 p.m. |
| Sat. June 5 | Miami | 7:00 p.m. |
| Sat. June 12 | at San Jose | 10:30 p.m. |
| Sat. June 19 | New England | 7:00 p.m. |
| Sun. June 27 | Los Angeles | 1:00 p.m. |
| Wed. June 30 | at MetroStars | 7:00 p.m. |
| Sat. July 3 | Kansas City | 7:00 p.m. |
| Thu. July 8 | San Jose | 7:30 p.m. |
| Thu. July 22 | at Kansas City | 8:00 p.m. |
| Sun. July 25 | at Colorado | 9:00 p.m. |
| Thu. July 29 | at Miami | 7:30 p.m. |
| Sat. July 31 | New England | 7:00 p.m. |
| Sun. Aug. 8 | at D.C. United | 2:00 p.m. |
| Fri. Aug. 13 | Colorado | 7:30 p.m. |
| Wed. Aug. 18 | at New England | 7:30 p.m. |
| Sun. Aug. 22 | at Columbus | 4:00 p.m. |
| Fri. Aug. 27 | Columbus | 7:30 p.m. |
| Sat. Sept. 4 | at New England | 7:30 p.m. |
| Fri. Sept. 10 | D.C. United | 7:30 p.m. |
| Fri. Sept. 17 | at Chicago | 7:30 p.m. |
| Sat. Sept. 25 | MetroStars | 7:00 p.m. |
| Wed. Oct. 6 | at D.C. United | 7:30 p.m. |
| Sun. Oct. 10 | Dallas | 8:00 p.m. |