MLS teams had to get their rosters down to 18 players April 15. They are also allowed to carry two developmental players who can train with the team. Players training with the U.S. Olympic team or on injured reserve don’t count against the limit.
Key: * = developmental player. ** = injured reserve. # = Olympic player.
Colorado Rapids
| No. | Goalkeepers | Last Club |
| 1 | Khalil Azmi | New Hampshire Ramblers |
| 18 | Dusty Hudock | Seattle Sounders |
| – | *Paul Grafer | William & Mary |
| No. | Defenders | Last Club |
| 4 | Steve Trittschuh | Tampa Bay Terror |
| 5 | Denis Hamlett | Anaheim Splash |
| 6 | Ian Butterworth | Norwich (England) |
| 7 | Troy Edwards | Myrtle Beach Boyz |
| 12 | Matt Kmosko | Cincinnati Silverbacks |
| 15 | **Scott Cannon | Cincinnati Silverbacks |
| 16 | Brian Bates | Richmond Kickers |
| 17 | Marcelo Balboa | Leon (Mexico) |
| No. | Midfielders | Last Club |
| 2 | Felipe Rodriguez | Standard Falcons |
| 8 | Dominic Kinnear | Seattle Sounders |
| 19 | Chris Henderson | FSV Frankfurt (Germany) |
| No. | Strikers | Last Club |
| 3 | Richard Sharpe | Cocoa Expos |
| 9 | Shaun Bartlett | Cape Town Spurs (So. Africa) |
| 10 | Roy Wegerle | Coventry City (England) |
| 13 | Jean Harbor | Seattle SeaDogs |
| 14 | Scott Benedetti | Colorado Foxes |
Columbus Crew
| No. | Goalkeepers | Last Club |
| 0 | **Kevin East | Jersey Dragons |
| 1 | Bo Oshoniyi | New York Centaurs |
| 18 | David Winner | Tampa Bay Cyclones |
| No. | Defenders | Last Club |
| 3 | Michael Clark | Richmond Kickers |
| 5 | Paul Caligiuri | St. Pauli (Germany) |
| 7 | Shane Battelle | St. Louis Knights |
| 8 | Brian Bliss | Carl Zeiss Jena (Germany) |
| 13 | Mark Watson | Vancouver 86ers |
| No. | Midfielders | Last Club |
| 4 | Janusz Michallik | New York Centaurs |
| 6 | Todd Yeagley | Richmond Kickers |
| 10 | #Brian Maisonneuve | Indiana Univ. |
| 11 | Adrian Paz | Ipswich (England) |
| 14 | Brandon Ward | Hampton Roads Mariners |
| 15 | Doctor Khumalo | Kaizer Chiefs (So. Africa) |
| 16 | #Rob Smith | Delaware Wizards |
| 17 | Paul Young | Charleston Battery |
| – | *Todd Miller | Westminster Coll. |
| No. | Strikers | Last Club |
| 2 | Mac Cozier | UNC Charlotte |
| 9 | Billy Thompson | Tampa Bay Terror |
| 12 | Pete Marino | Bayer Leverkusen (Germany) |
| 20 | Brian McBride | Wolfsburg (Germany) |
| 22 | Marcelo Carrera | Buffalo Blizzard |
| – | *Ricci Greenwood | Seattle Pacific |
Dallas Burn
| No. | Goalkeepers | Last Club |
| 00 | #Jeff Cassar | Florida International |
| 1 | Mark Dodd | Colorado Foxes |
| – | **Jon Walker | Louisville Univ. |
| No. | Defenders | Last Club |
| 3 | Ed Puskarich | Milwaukee Wave |
| 6 | **Tom Soehn | Wichita Wings |
| 11 | Lawrence Lozzano | Tam. Madero (Mexico) |
| 18 | Richard Farrer | New Mexico Chiles |
| 22 | Diego Sonora | Boca Juniors (Argentina) |
| 26 | #Brandon Pollard | Univ. Virginia |
| – | *Juan Satoque | — |
| No. | Midfielders | Last Club |
| 4 | Chad Ashton | Wichita Wings |
| 5 | Jorge Flores | — |
| 7 | Mark Santel | St. Louis Ambush |
| 8 | Gerell Elliott | Sacramento Knights |
| 10 | W. Rodriguez | Liverpool (Uruguay) |
| 13 | Brian Haynes | Kansas City Attack |
| 14 | Leonel Alvarez | America Cali (Colombia) |
| No. | Strikers | Last Club |
| 9 | Hugo Sanchez | Linz (Austria) |
| 12 | Ted Eck | Wichita Wings |
| 15 | Jason Kreis | Raleigh Flyers |
| 17 | John Kerr Jr. | Walsall (England) |
| 19 | Jimmy Glenn | Cincinnati Silverbacks |
Kansas City Wiz
| No. | Goalkeepers | Last Club |
| 0 | #Chris Snitko | UCLA |
| 1 | Pat Harrington | Buffalo Blizzard |
| 25 | Garth Lagerwey | Hampton Roads Mariners |
| No. | Defenders | Last Club |
| 2 | Tom Reasoner | San Diego Top Guns |
| 4 | Scott Uderitz | Las Vegas Dustdevils |
| 6 | Sean Bowers | Anaheim Splash |
| 15 | Samuel Ekeme | Monterrey La Raza |
| 20 | Uche Okafor | Ironi Ashdod (Israel) |
| No. | Midfielders | Last Club |
| 10 | Mike Sorber | UNAM (Mexico) |
| 11 | Preki | San Jose Grizzlies |
| 13 | Mark Chung | San Diego Sockers |
| 16 | Diego Gutierrez | Rockhurst Coll. |
| 17 | Kevin Koetters | Kansas City Attack |
| 18 | *Bill Baumhoff | St. Louis Knights |
| 21 | #Matt McKeon | Saint Louis Univ. |
| No. | Strikers | Last Club |
| 3 | **Paul Wright | Sacramento Knights |
| 7 | *Ayo Peters | Locarno (Switzerland) |
| 8 | Eric Eichmann | St. Louis Storm |
| 9 | Peter Isaacs | Tampa Bay Cyclones |
| 12 | Vitalis Takawira | Dynamos (Zimbabwe) |
| 14 | Frank Klopas | Apollon (Greece) |
| 23 | Alan Prampin | Dallas Sidekicks |
Los Angeles Galaxy
| No. | Goalkeepers | Last Club |
| 1 | David Kramer | Monterey Bay Jaguars |
| 9 | Jorge Campos | Atlante (Mexico) |
| No. | Defenders | Last Club |
| 3 | Mark Semioli | Monterey Bay Jaguars |
| 4 | Robin Fraser | Colorado Foxes |
| 5 | Jorge Salcedo | Morelia (Mexico) |
| 6 | Dan Calichman | New York Centaurs |
| 15 | Manny Motajo | Washington Warthogs |
| 20 | Curt Onalfo | Tam. Madero (Mexico) |
| No. | Midfielders | Last Club |
| 7 | **Andrew Shue | Los Angeles United |
| 8 | Brad Wilson | Saarbruecken (Germany) |
| 10 | Mauricio Cienfuegos | Luis Angel Firpo (El Salvador) |
| 13 | Cobi Jones | Vasco da Gama (Brazil) |
| 14 | Chris Armas | Long Island Rough Riders |
| 18 | *Greg Vanney | UCLA |
| 19 | Arash Noamouz | Houston Hotshots |
| No. | Strikers | Last Club |
| 12 | *Guillermo Jara | Univ. San Diego |
| 17 | Jose Vasquez | Celaya (Mexico) |
| 21 | John Garvey | Baltimore Spirit |
| 22 | Joey Kirk | St. Louis Ambush |
| 29 | Eduardo Hurtado | Colo Colo (Chile) |
New England Revolution
| No. | Goalkeepers | Last Club |
| 1 | Jim St. Andre | New York Centaurs |
| 31 | Jim Adams | Cleveland Crunch |
| No. | Defenders | Last Club |
| 2 | Zak Ibsen | Tampa Bay Terror |
| 3 | **Iain Fraser | Sacramento Knights |
| 5 | Ted Chronopoulos | Panionios (Greece) |
| 12 | Richard Weiszmann | North Bay Breakers |
| 16 | Tom Lips | New York Fever |
| 22 | Alexi Lalas | Padova (Italy) |
| No. | Midfielders | Last Club |
| 4 | Mike Burns | Viborg (Denmark) |
| 6 | Geoff Aunger | Milwaukee Wave |
| 8 | Peter Woodring | Hawaii Tsunami |
| 10 | Alberto Naveda | Boca Juniors (Argentina) |
| 14 | John DeBrito | New York Fever |
| 17 | *Paulo Dos Santos | Univ. Rhode Island |
| 21 | #Imad Baba | Clemson Univ. |
| No. | Strikers | Last Club |
| 7 | Darren Sawatzky | Cascade Surge |
| 9 | Giuseppe Galderisi | Padova (Italy) |
| 11 | Welton | America (Brazil) |
| 18 | Paul Keegan | Boston Coll. |
| 19 | Rob Ukrop | Richmond Kickers |
| 24 | Bojan Vukovic | Tampa Bay Terror |
MetroStars
| No. | Goalkeepers | Last Club |
| 1 | Tony Meola | Long Island Rough Riders |
| 18 | #Zach Thornton | Loyola (Md.) Coll. |
| No. | Defenders | Last Club |
| 2 | Jeff Zaun | North Jersey Imperials |
| 3 | Matt Knowles | Milwaukee Wave |
| 5 | Nicola Caricola | Torino (Italy) |
| 16 | Chris Da Silva | Jersey Dragons |
| 20 | Andrew Restrepo | Tampa Bay Cyclones |
| No. | Midfielders | Last Club |
| 6 | #Damian Silvera | Univ. Virginia |
| 7 | Roberto Donadoni AC Milan (Italy) | |
| 10 | Tab Ramos | UNL (Mexico) |
| 11 | Ed Rodriguez | Tampa Bay Terror |
| 12 | *Ted Gillen | Richmond Kickers |
| 13 | Dan Barber | Las Vegas Dustdevils |
| 14 | Mickey Kydes | New York Fever |
| 15 | Rhett Harty | San Jose Grizzlies |
| 21 | *Nidal Baba | Clemson Univ. |
| No. | Strikers | Last Club |
| 9 | Peter Vermes | New York Fever |
| 17 | Giovanni Savarese | Long Island Rough Riders |
| 19 | #Miles Joseph | Detroit Rockers |
| 22 | #A.J. Wood | Univ. Virginia |
San Jose Clash
| No. | Goalkeepers | Last Club |
| 1 | Tom Liner | PSV Schwartz Weiss (Austria) |
| 1 | Dave Salzwedel | S.F. Bay Blackhawks |
| No. | Defenders | Last Club |
| 2 | Oscar Draguicevich | Cleveland Crunch |
| 3 | John Doyle | Atlanta Ruckus |
| 4 | **Rafael Amaya | Colorado Foxes |
| 5 | Michael Emenalo | Notts County (England) |
| 12 | Troy Dayak | Detroit Rockers |
| 17 | Tim Martin | Atlanta Ruckus |
| No. | Midfielders | Last Club |
| 6 | Ben Iroha | Vitesse (Netherlands) |
| 9 | Paul Bravo | Monterey Bay Jaguars |
| 10 | Victor Mella | Colo Colo (Chile) |
| 14 | Paul Holocher | Monterey Bay Jaguars |
| 15 | Altimont Butler | Jersey Dragons |
| 18 | Henry Gutierrez | Cleveland Crunch |
| 23 | Jorge Rodas | Comunicaciones (Guatemala) |
| No. | Strikers | Last Club |
| 8 | Jeff Baicher | San Jose Grizzlies |
| 11 | Eric Wynalda | Bochum (Germany) |
Tampa Bay Mutiny
| No. | Goalkeepers | Last Club |
| 1 | Mark Dougherty | Hawaii Tsunami |
| 22 | Scott Budnick | Richmond Kickers |
| No. | Defenders | Last Club |
| 2 | John Diffley | Long Island Rough Riders |
| 3 | Steve Pittman | Partick Thistle (Scotland) |
| 4 | Cle Kooiman | Morelia (Mexico) |
| 5 | Frank Yallop | Blackpool (England) |
| 12 | Adam Frye | UCLA |
| 13 | Frankie Hejduk | UCLA |
| 20 | Nelson Vargas | — |
| – | Mike DuHaney | UNLV |
| – | **Tayt Ianni | Rajpracha (Thailand) |
| No. | Midfielders | Last Club |
| 6 | Martin Vasquez | Atlas (Mexico) |
| 9 | **Peter Hattrup | Seattle Sounders |
| 10 | Carlos Valderrama | Atl. Junior (Colombia) |
| 14 | Steve Ralston | St. Louis Storm |
| 16 | Derek Backman | Tampa Bay Cyclones |
| 17 | **Greg Lalas | Brown Univ. |
| – | Goran Hunjak | Kansas City Attack |
| No. | Strikers | Last Club |
| 8 | Ivan McKinley | Wits Univ. (South Africa) |
| 11 | Roy Lassiter | Alajuela (Costa Rica) |
| 18 | Diego Viera | Cerro (Uruguay) |
| 19 | Evans Wise | Egelsbach (Germany) |
Washington D.C. United
| No. | Goalkeeper | Last Club |
| 26 | Jeff Causey | Richmond Kickers |
| No. | Defenders | Last Club |
| 4 | #Clint Peay | Univ. Virginia |
| 5 | Erik Imler | Raleigh Stars |
| 7 | Kris Kelderman | Milwaukee Wave |
| 11 | Mario Gori | Rosario Central (Argentina) |
| 12 | Jeff Agoos | Wehen (Germany) |
| 23 | #Eddie Pope | Univ. North Carolina |
| No. | Midfielders | Last Club |
| 6 | John Harkes | West Ham (England) |
| 8 | Brian Kamler | Richmond Kickers |
| 10 | Marco Etcheverry | Colo Colo (Chile) |
| 13 | Shawn Medved | Cincinnati Silverbacks |
| 16 | Richie Williams | Richmond Kickers |
| 18 | Mike Huwiler | Atlanta Ruckus |
| No. | Strikers | Last Club |
| 9 | Juan Berthy Suarez | Guabira (Bolivia) |
| 15 | *Jesse Marsch | Princeton Univ. |
| 19 | Sterling Wescott | Wichita Wings |
| 21 | Raul Diaz Arce | Luis Angel Firpo (El Salvador) |
| 22 | Ben Crawley | Richmond Kickers |